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pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222858

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CAG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCAG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222860

Purpose

This plasmid codes for the Slug-KH nickase with a sg(GCA)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgGCA

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD ATP6-2-1

Plasmid

#210829

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD nad7-2

Plasmid

#210832

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD nad7-1

Plasmid

#210831

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD ATP6-2-2

Plasmid

#210830

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Bneu-10

Plasmid

#205215

Purpose

Unit Bactoneuron-10, expresses E2-crimson

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Inserts

E2-Crimson
LuxR

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM010_P5_ConR9

Plasmid

#216435

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R9

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM009_P5_ConR8

Plasmid

#216434

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R8

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM008_P5_ConR7

Plasmid

#216433

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R7

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM007_P5_ConR6

Plasmid

#216432

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R6

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM004_P1_ConL9

Plasmid

#216429

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L9

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM003_P1_ConL8

Plasmid

#216428

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L8

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM002_P1_ConL7

Plasmid

#216427

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L7

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM001_P1_ConL6

Plasmid

#216426

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L6

Use

Synthetic Biology

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-paGFP-bPAC

Plasmid

#204670

Purpose

Mammalian expression of bPAC tagged with paGFP for cAMP production

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Insert

paGFP-bPAC

Use

Lentiviral

Tags

10xHis and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBP_dCas9

Plasmid

#190131

Purpose

Level 0 MoClo plasmid containing dCas9

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-RiboJ-BCD2-E0040m-B0015)

Plasmid

#197673

Purpose

Expresses GFP under the control of J23106 and RiboJ-BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23103-RiboJ-UTR1-E0040m-B0015)

Plasmid

#197668

Purpose

Expresses GFP under the control of J23103 and RiboJ-UTR1

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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p5418

Plasmid

#168185

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PtetA::dsred PcypD::sfgfp (frameshift)

Expression

Bacterial

Mutation

frameshift sfGFP

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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