Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,349 results for: 221

Showing: 681 - 700 of 8349 results

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pRetroX-TRE3G-GMIP P564D

Plasmid

#221324

Purpose

Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P564D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-GMIP S31D

Plasmid

#221322

Purpose

Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S31D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 P278D P281D

Plasmid

#221320

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P278D P281D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-GMIP

Plasmid

#221318

Purpose

Dox-inducible expression of GMIP in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-NOSTRIN

Plasmid

#221309

Purpose

Expression of NOSTRIN (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

NOSTRIN (pLxIS) (Nostrin Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-ASAP3(Cterm)

Plasmid

#221304

Purpose

Expression of ASAP3 (Cterm pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ASAP3 (Cterm pLxIS) (Asap3 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-ASAP2

Plasmid

#221303

Purpose

Expression of ASAP2 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ASAP2 (pLxIS) (Asap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-ARHGAP45

Plasmid

#221301

Purpose

Expression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

ARHGAP45 (pLxIS) (Hmha1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-p27(pLxIS-PLPLR)

Plasmid

#221286

Purpose

Expression of p27 (pLxIS-PLPLR) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

p27 (pLxIS-PLPLR) (Cdkn1b Synthetic)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* ectodomain

Plasmid

#221399

Purpose

Mammalian expression of human integrin beta1 Q280* K418* ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRSp53(trunc N1)

Plasmid

#221272

Purpose

Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 N-terminal truncation 1 (257-375) (Baiap2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N562* full-length

Plasmid

#221405

Purpose

Mammalian expression of human integrin beta1 N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-MAVS(pLxIS) E433A

Plasmid

#221258

Purpose

Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS (393-441) (Mavs Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Mutation

E433A

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FKBP-TRIF(trunc 1-183)

Plasmid

#221255

Purpose

Expression of TRIF truncation 9 (184-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 9 (184-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-FKBP-MAVS(pLxIS)

Plasmid

#221335

Purpose

Overexpression of MAVS pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS domain (Mavs Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 681 - 700 of 8349 results

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