We narrowed to 7,368 results for: ALP

1. Stat1 alpha pRC/CMV

   Plasmid

   #8690

   Depositor

   Jim Darnell

   Article

   Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. FusX alphaDdCBE DddA6-11 1397C

   Plasmid

   #221938

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FusX alphaDdCBE DddA6 1397N

   Plasmid

   #221939

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FusX alphaDdCBE DddA11 1397N

   Plasmid

   #221940

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1alpha-soCoChR-tdTomato

   Plasmid

   #107714

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-tdTomato
   

   Use

   AAV

   Tags

   KA2 and tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-PDGFRalpha-V242K

   Plasmid

   #136436

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   V242K (disrupts PDGF binding, but still binds HCM…

   Promoter

   CMV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

   Plasmid

   #160607

   Purpose

   TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

   Depositor

   Diego Orzaez

   Insert

   CUP2Gal4AD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2-sgAMPK alpha1 clone1

   Plasmid

   #162119

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

   Plasmid

   #192651

   Purpose

   3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-10xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LMP AMPK alpha1 hp1

    Plasmid

    #89489

    Purpose

    shRNA against AMPK alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid

    #88879

    Purpose

    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

    Insert

    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP fusion protein and HA tag

    Expression

    Mammalian

    Mutation

    amino acids 4-460 only; L78P & L287P *see bel…

    Promoter

    SFFV (spleen focus forming virus)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-hSyn-Galpha-s ONE-GO

    Plasmid

    #214371

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLVX-hSyn-Galpha-q ONE-GO

    Plasmid

    #204336

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-q ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLVX-hSyn-Galpha-13 ONE-GO

    Plasmid

    #204337

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-13 ONE-GO (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

    Plasmid

    #66789

    Purpose

    Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D842V (constitutively active)

    Promoter

    EF1a

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBSSK WT p85 alpha flag

    Plasmid

    #13429

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (PIK3R1 Human)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid

    #221997

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge