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Plasmid#202105PurposeLevel 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertBCA promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-epyC
Plasmid#202104PurposeLevel 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertepyC promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove bsaI sites fo…PromoterAvailable sinceAug. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM1-zeo E321K ORF29
Plasmid#200025PurposeLentiviral vector for expression of KSHV ORF29 with E321K mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlutamic acid 321 changed to lysinePromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo G226A E321K ORF29
Plasmid#200026PurposeLentiviral vector for expression of KSHV ORF29 with G226A and E321K mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationGlycine 226 changed to alanine, glutamic acid 321…PromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo D476A ORF29
Plasmid#200027PurposeLentiviral vector for expression of KSHV ORF29 with D476A mutationDepositorInsertORF29
UseLentiviralTagsExpressionMutationAspartic acid 476 changed to AlaninePromoterCMVAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBImut-T2A-caMEK
Plasmid#199579PurposeExpress tKiMBImut(AA) and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Mm_Plk5(D168A)
Plasmid#136318PurposeMammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFPDepositorInsertPLK5 (Plk5 Mouse)
UseTagsExpressionMammalianMutationD168APromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Hu_Plk5(W207F)
Plasmid#136320PurposeMammalian expression of human Plk5 W207F with N-terminal EGFPDepositorInsertPLK5 (PLK5 Human)
UseTagsExpressionMammalianMutationW207FPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
UseTagsExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111923PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μDepositorInsertCeNL(Ca2+)_19μ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111922PurposeCyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μDepositorInsertCeNL(Ca2+)_1.2μ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205097Purposefusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PtPuc3_diaCas9_sgRNA
Plasmid#109219PurposeEpisome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutumDepositorInsertsLHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterLHCF1 terminator, LHCF2 promoter, U6 3' regi…Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNAZeo(-) LAMP2C
Plasmid#89342PurposeExpresses human LAMP-2C in mammalian cellsDepositorInsertLysosomal-associated membrane protein 2C (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBP_2xNbLYS1::LUZ
Plasmid#189918PurposeBinary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.DepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromoterVariousAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205098PurposeCre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKL9h, and Volvox channelrhodopsin-1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Mm_CEP55(WT)
Plasmid#136333PurposeMouse CEP55 cDNA in Gateway pENTRDepositorInsertCEP55 (Cep55 Mouse)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)
Plasmid#114102Purposefusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGLucM23-iChloC-EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only