We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1 Mfn2-HA S27A

   Plasmid

   #139193

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Alanine

   Promoter

   CMV promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Mfn2-HA S27D

   Plasmid

   #139194

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Aspartic Acid

   Promoter

   CMV promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-ERBB4-V5/HIS

   Plasmid

   #201105

   Purpose

   expression of human ERBB4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. DRD2-3xHA/pCDNA3.1

   Plasmid

   #196940

   Purpose

   Plasmid expressing an antigen targeting the D2 dopamine receptor

   Depositor

   James Trimmer

   Insert

   DRD2 (DRD2 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-HA-FbxO11-pcDNA3.1-

   Plasmid

   #52501

   Purpose

   expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(-)-qmTQ2-ATP

   Plasmid

   #225248

   Purpose

   mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His-Precission-NEDD4L_FL_pCDNA3.1(+)

   Plasmid

   #187730

   Purpose

   Express NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-iBB-GECO1

   Plasmid

   #194215

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

   Depositor

   Jin Zhang

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Pur-mEGFP

   Plasmid

   #186329

   Purpose

   Fluorescent reporter system

   Depositor

   Bertrand Collet

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

    Plasmid

    #131034

    Purpose

    Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRB (Tcrb Mouse)
    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. K44A HA-dynamin 1 pcDNA3.1

    Plasmid

    #34683

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-ccdB-3xHA

    Plasmid

    #214986

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-ExRai-AKAR

    Plasmid

    #118405

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-VEGFR2-mCherry

    Plasmid

    #139606

    Purpose

    Expresses a VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-HA-mSTING (V154M)

    Plasmid

    #214159

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V154M) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-N-Myc_C223S USP7

    Plasmid

    #131243

    Purpose

    Mammalian expression of catalytically inactive Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 cyst…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-AKAP95-FRB

    Plasmid

    #138254

    Purpose

    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    AKAP95-FRB (AKAP8 Human)
    

    Use

    Tags

    FRB rapamycin binding domain and HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-Elongin B-pcDNA3.1(+)-Zeo

    Plasmid

    #20000

    Depositor

    William Kaelin

    Article

    Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

    Insert

    transcription elongation factor B (ELOB Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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