We narrowed to 8,696 results for: SET
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Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
ExpressionBacterialPromoterpBADAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…PromoterContain GG cassette and MCS with LacZ for blue/wh…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1033_v1
Plasmid#126860Purpose4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1033_v1
UseSynthetic BiologyAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v3
Plasmid#158030Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/BFP Dest
Plasmid#74282PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloningDepositorInsertRosa26 5-homology region
Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterJ23115 and T7/lacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-TWIST2
Plasmid#197935PurposeGateway entry vector for an inducible 3xFLAG-tagged TWIST2DepositorAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC65
Plasmid#118865Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFPExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-RFP-HYGRO
Plasmid#115145PurposeSafe harbor site 231 knock-in vector with RFP-HygromycinR expression cassetteDepositorInsertRFP
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSNK
Plasmid#135193PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC102
Plasmid#118883Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallin and cmlc2Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIFV
Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only