Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 14,240 results for: cas9

Clear keywords
Showing: 6981 - 7000 of 14240 results

  2. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

    Enlarge

  3. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

    Enlarge

  4. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid
    #115189
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115189-plasmid-map-sequence-id-225429

    Enlarge

  5. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid
    #115190
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115190-plasmid-map-sequence-id-225431

    Enlarge

  6. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid
    #115191
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115191-plasmid-map-sequence-id-225432

    Enlarge

  7. pORFE1001

    Plasmid
    #112079
    Purpose
    Overexpression of the AtCAS9 in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    2x35S
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112079-plasmid-map-sequence-id-217610

    Enlarge

  8. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

    Enlarge

  9. pRNF219.1.0-gDNA

    Plasmid
    #132464
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RNF219
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132464-plasmid-map-sequence-id-267084

    Enlarge

  11. pRAC10A

    Plasmid
    #193734
    Purpose
    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193734-plasmid-map-sequence-id-381905

    Enlarge

  12. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

    Enlarge

  19. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

    Enlarge

  20. pKb-TnpB1

    Plasmid
    #215333
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and OsU3 promote…
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    215333-plasmid-map-sequence-id-446611

    Enlarge

Showing: 6981 - 7000 of 14240 results