164,722 results

1. pGD-P1/HC-Pro

   Plasmid

   #196328

   Purpose

   35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tobacco etch virus P1/HC-Pro
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPA-GFP-CD81-10

   Plasmid

   #57124

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pcDNA3.1(+)/Luc2=tdT

   Plasmid

   #32904

   Purpose

   Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

   Depositor

   Christopher Contag

   Article

   Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

   Inserts

   Firefly Luciferase
   tandem Tomato Red Fluorescent Protein
   

   Use

   Tags

   tdTomato red fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and n/a

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-tmpknot-epegRNA-puro backbone

   Plasmid

   #214089

   Purpose

   Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Mitochondrial PancACe

   Plasmid

   #215710

   Purpose

   Expresses acetyl-CoA biosensor in the mitochondria

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and mitochondrial localization sequence…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-MCS1-P2A-MCS2-mDux

   Plasmid

   #138320

   Purpose

   Dox-inducible codon-optimized mouse Dux

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   Dux (Dux Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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