We narrowed to 18,489 results for: Met

1. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

   Plasmid

   #175530

   Purpose

   Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CalR-HaloTag7-KDEL
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB501NL3F10H

   Plasmid

   #141286

   Purpose

   No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Type

   Empty backbone

   Use

   Luciferase; Gateway

   Tags

   FLAG (x3), HIS (x10), and NLUC

   Expression

   Plant

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11a-SUMO3

   Plasmid

   #53143

   Purpose

   E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   SUMO3 (SUMO2 Human)
   

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-93)

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA-ROSA26_hspCas9

   Plasmid

   #193310

   Purpose

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_HaloTag9_LamB1

   Plasmid

   #175538

   Purpose

   Expression of HaloTag9 at the nuclear lamina of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9-LamB1
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_Lyn11-HaloTag9

   Plasmid

   #175543

   Purpose

   Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lyn11-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. BFP dest clone

   Plasmid

   #71825

   Purpose

   Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCT62

   Plasmid

   #170391

   Purpose

   expresses three sgRNA cassettes

   Depositor

   Jonathan Weissman

   Article

   Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

   Insert

   PCT62-targetsite
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-LSSmGFP

   Plasmid

   #186542

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   araB

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL Blast mouse E-cadherin

    Plasmid

    #18804

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. JunB

    Plasmid

    #187903

    Purpose

    Expresses JunB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    JunB (Junb Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICSL1P1_AtU626Ter26

    Plasmid

    #191769

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-G-Cdc42

    Plasmid

    #180577

    Purpose

    ddGFP sensor of Cdc42

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Cdc42
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKIKOarsBKm

    Plasmid

    #46766

    Purpose

    E.coli genomic integration at arsB

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLH-sgRNA-Sirius-8XMS2

    Plasmid

    #121939

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Flag RbBP5

    Plasmid

    #15550

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    RbBP5 (RBBP5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pSR prdm3 (PRDM16 shRNA)

    Plasmid

    #15505

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 shRNA (Prdm16 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pBABE-puro-mTwist

    Plasmid

    #1783

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    Twist (Twist1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET11a-SUMO2

    Plasmid

    #53142

    Purpose

    E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO2 (SUMO3 Human)
    

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-92)

    Promoter

    T7

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_Lyn11-HaloTag7

    Plasmid

    #175533

    Purpose

    Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge