We narrowed to 7,206 results for: RAP

1. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Use

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 HA-FAM208A

   Plasmid

   #115845

   Purpose

   Encodes codon optimized HA tagged FAM208A

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HA tagged FAM208A (C3orf63 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAPM-D4 miR30-FAM208A ts2

   Plasmid

   #115872

   Purpose

   FAM208a knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   FAM208a shRNA (C3orf63 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX-SHP1C/S-ires-mCD8

   Plasmid

   #183719

   Purpose

   Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mutation

   C453S

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAPM-D4 miR30-FAM208A ts3

   Plasmid

   #115873

   Purpose

   FAM208a knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   FAM208a shRNA (C3orf63 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Use

   Tags

   3x HA, V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-PdTK-CAG-mCh-[uBglII]

   Plasmid

   #107280

   Purpose

   puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAPM-D4 miR30-FAM208A ts1

   Plasmid

   #115871

   Purpose

   FAM208a knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   FAM208a shRNA (C3orf63 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-eYFP-FKBP-MAD1-WT

    Plasmid

    #114037

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-C1-FKBP-CYB5tail

    Plasmid

    #202686

    Purpose

    rapamycin-induced recruitment of proteins to ER

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd5-Blue

    Plasmid

    #174431

    Purpose

    pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.

    Depositor

    Teresa De Los Santos

    Article

    Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.

    Insert

    β-galactosidase α gene fragment
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-ANLN_sgRNA

    Plasmid

    #183874

    Purpose

    pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LBR-FKBP-GFP

    Plasmid

    #222413

    Purpose

    To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1846

    Plasmid

    #154326

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP^SEC^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCYC1pr-GNSI (LEU)

    Plasmid

    #111450

    Purpose

    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA1_IL1RN

    Plasmid

    #64140

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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