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pLenti6.3_CRKL-WT-V5

Plasmid

#196018

Purpose

Expresses CRKL WT, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Publication

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEY26

Plasmid

#191031

Purpose

cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.3-2.6k) (cho-1 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.CAG-pHlu

Plasmid

#129331

Purpose

pH measurement, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CDreV

Plasmid

#140133

Purpose

can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Publication

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CDreV

Use

AAV

Promoter

EF1a

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Publication

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Expression

Mammalian

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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SRF WT pGEX4T

Plasmid

#121100

Purpose

Expression of SRF as a GST fusion protein

Depositor

Paul Herring

Publication

Herring et al J Biol Chem. 2001 Apr 27;276(17):14482-9

Insert

SRF (SRF Human)

Tags

GST

Expression

Bacterial

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p15A_J23108_SpydCas9_CmR

Plasmid

#119163

Purpose

Expresses S. pyogenes dCas9

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9 from S. pyogenes

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pJA59

Plasmid

#59932

Purpose

gRNA for cleavage at unc-109(n499) locus in C elegans

Depositor

Andrew Fire

Publication

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-109(n499) gRNA

Use

CRISPR

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGR-ECK125108723

Plasmid

#46008

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK125108723 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-ECK120010783

Plasmid

#46009

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120010783 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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KaiB-FLAG

Plasmid

#41885

Depositor

Michael Rust

Publication

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai B

Tags

FLAG

Expression

Bacterial

Promoter

t7 promoter

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pLX-EF1a_mCherry

Plasmid

#221482

Purpose

mCherry lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

mCherry

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG416GAL-EGFP

Plasmid

#166140

Purpose

EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMUT2-pFF-41.5-mCherry

Plasmid

#192863

Purpose

pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

Depositor

Morten Sommer

Publication

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Oxygen inducible promoter

Expression

Bacterial

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Publication

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EYFP

Plasmid

#167858

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CDS12_mTurquoise2

Plasmid

#136089

Purpose

FP for N-term fusion with a CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-SYNZIP11

Plasmid

#80666

Purpose

Encodes the synthetic coiled coil peptide SYNZIP11

Depositor

Amy Keating

Publication

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP11

Use

Gateway Cloning

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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