We narrowed to 19,084 results for: iNOS

1. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

   Plasmid

   #203447

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTag4C_AVPR1A-V2R-NTEV-tcs-GV

   Plasmid

   #227102

   Purpose

   AVPR1A receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   AVPR1A-NTEV (AVPR1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTag4C_ADRA1A-V2R-NTEV-tcs-GV

   Plasmid

   #227101

   Purpose

   ADRA1A receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   ADRA1A-NTEV (ADRA1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_GR-RE_MLPmin_BC1093-luc2

   Plasmid

   #227113

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBK_Gal4-DBD-ESR1

   Plasmid

   #227099

   Purpose

   ESR1 receptor overexpression for luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCrhaB2_ApyD_ RBS_ApyAOHIS

   Plasmid

   #226247

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   ApyH
   ApyI
   ApyS
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

   Plasmid

   #215910

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332D, S1346D, S1616D, S169…

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

   Plasmid

   #215911

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   Flag-HA-SBP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A, S1616A, S169…

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

   Plasmid

   #215905

   Purpose

   Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

   Depositor

   Kumiko Ui-Tei

   Article

   Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   contain aa1224-1709, S1332A, S1346A, S1616A, S169…

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-dSV40-CD80-mApple

    Plasmid

    #223595

    Purpose

    Lentiviral expression of human CD80 with mApple in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mApple

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-dSV40-CD80-mGFP

    Plasmid

    #223592

    Purpose

    Lentiviral expression of human CD80 with mGFP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-SNAP-CD80 (I92R)

    Plasmid

    #223589

    Purpose

    Lentiviral expression of human CD80 (I92R) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    I92R

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPPI4-SNAP-CTLA-4-His6

    Plasmid

    #223584

    Purpose

    Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPPI4-Strep-SNAP-CD80

    Plasmid

    #223581

    Purpose

    Protein purification of human CD80 with Twin-Strep and SNAP tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid

    #191572

    Purpose

    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    D614G and F486S (confers reduced binding affinity…

    Promoter

    CMV

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-EF1A-Puro-mCherry-RAB7-S72A

    Plasmid

    #221556

    Purpose

    Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Change serine 72 to alanine

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA320

    Plasmid

    #215952

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA315

    Plasmid

    #215951

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge