We narrowed to 7,609 results for: Pol

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WNV NS2B-NS3 protease: Co-expression of NS2B and NS3

Plasmid

#204806

Purpose

Bacterial co-expression of West Nile NS2B and NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B

Expression

Bacterial

Available Since

Dec. 18, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac1-bullfrog saxiphilin

Plasmid

#140595

Purpose

protein expression

Depositor

Dan Minor

Article

Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

Insert

saxiphilin bullfrog

Tags

GFP-His10

Expression

Insect

Mutation

Deletion of M20 - please see depositor comment

Promoter

polyhedrin promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S172A_M173A RAD23A

Plasmid

#201574

Purpose

Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutations S172A and M173A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V195A RAD23A

Plasmid

#201575

Purpose

Expresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V195A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_C344A RAD23A

Plasmid

#201577

Purpose

Expresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation C344A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F354A RAD23A

Plasmid

#201578

Purpose

Expresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F354A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_C344A_F354A RAD23A

Plasmid

#201579

Purpose

Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutations C344A and F354A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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tl1-FusXTBE-RightTALE

Plasmid

#196507

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-Cenexin-S796A

Plasmid

#186434

Purpose

Expresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

Insert

Cenexin (ODF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed Serine 796 to Alanine

Promoter

CMV IE94

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

Plasmid

#174131

Purpose

Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized, H163A, H258A

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBST

Plasmid

#172968

Purpose

pWSK29 with Salmonella typhimurium wzzBST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

K198E- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-fepEST

Plasmid

#172969

Purpose

pWSK29 with Salmonella typhimurium fepEST gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

fepE gene from Salmonella enterica serovar Typhimurium

Expression

Bacterial

Mutation

G160S- please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37I-A44L

Plasmid

#162583

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

A37I and A44L

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNG1304

Plasmid

#162488

Purpose

Expresses HelD K239A from Bacillus subtillis

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

HelD (helD Bacillus subtillis 168)

Tags

6His-V5

Expression

Bacterial

Mutation

Changed Lysine 239 to Alanine (K239A)

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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