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Addgene

We narrowed to 50,410 results for: ANG

Showing: 7041 - 7060 of 50410 results
  1. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid
    #158576
    Purpose
    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Insert
    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    Use
    Tags
    V5 and HA on the endogenous KCC2 protein product
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pTT5-hSDC1-CS2

    Plasmid
    #52367
    Purpose
    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R95Q and S194A (according to numbering in Fig. 2A…
    Promoter
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3-InPTapp-Kras

    Plasmid
    #235999
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.
    Depositor
    Insert
    InPTapp-Kras (PLEKHA1 Synthetic, Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-InPGrp-Kras

    Plasmid
    #236001
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.
    Depositor
    Insert
    InPGrp-Kras (Cyth3 Synthetic, Rat)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-Endo-InPGrp

    Plasmid
    #236003
    Purpose
    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.
    Depositor
    Insert
    Endo-InPGrp (Cyth3 Synthetic, Rat)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. RAT +D122H

    Plasmid
    #187426
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed aspartic acid at 129 for histidine
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. RAT +R111E

    Plasmid
    #187425
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed arginine at 118 to glutamic acid
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. S+10subs

    Plasmid
    #167176
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells
    Depositor
    Inserts
    ATP1A1-S+10R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed A at 112 to T; E at 116 to D; I at 135 to…
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 7041 - 7060 of 50410 results