We narrowed to 8,688 results for: App

1. pIGAhRC

   Plasmid

   #112510

   Purpose

   human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

   Depositor

   Charles Miller

   Article

   Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

   Insert

   HIF1B, AhR (ARNT, AHR Budding Yeast, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1,10

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL-Neo-Myr-Flag-DEST

   Plasmid

   #15300

   Purpose

   a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

   Depositor

   Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/T0-Flag-RECQL5

   Plasmid

   #222620

   Purpose

   Mammalian expression vector of flag-tagged RECQL5

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   RECQL5 (RECQL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

   Plasmid

   #224497

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

   Plasmid

   #187575

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgCrebbp#2/Cre

   Plasmid

   #193210

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgCrebbp#2 (Crebbp Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Myo10

    Plasmid

    #135403

    Purpose

    Expresses GFP-tagged bovine Myo10 in mammalian cells.

    Depositor

    Richard Cheney

    Article

    Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

    Insert

    Myo10 (MYO10 Bovine)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    "Relative to the GenBank Myo10 cDNA sequence…

    Promoter

    CMV

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

    Plasmid

    #122668

    Purpose

    C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 186-230

    Promoter

    SFFV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 SARS-CoV-2 NSP16

    Plasmid

    #141269

    Purpose

    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCMV-CIB1-mCerulean-MP

    Plasmid

    #58366

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR207 SARS-CoV-2 ORF3A

    Plasmid

    #141271

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDONR207 SARS-CoV-2 E

    Plasmid

    #141273

    Purpose

    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

    Plasmid

    #187581

    Purpose

    Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    COILIN (COIL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR207 SARS-CoV-2 NSP1

    Plasmid

    #141255

    Purpose

    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Mouse Ism1 with C-terminal Myc-6X-his tag

    Plasmid

    #173046

    Purpose

    Expresses Ism1 in mammalian cells

    Depositor

    Katrin Svensson

    Article

    Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

    Insert

    Ism1 (Ism1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR207 SARS-CoV-2 M

    Plasmid

    #141274

    Purpose

    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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