We narrowed to 11,713 results for: NSI

1. pMXs-hFOXH1GR

   Plasmid

   #59311

   Purpose

   Forced expression of human FOXH1GR

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

   Insert

   FOXH1 (FOXH1 Human)
   

   Use

   Retroviral

   Tags

   cGR - hormone binding domain of glucocorticoid re…

   Expression

   Mammalian

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS664-pMCSG7-AcrIIC5Smu

   Plasmid

   #113441

   Purpose

   Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   lacI

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b Me31B-3Myc

   Plasmid

   #113001

   Purpose

   For protein expression and purification of Myc-tagged full-length Drosophila me31B

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length me31B (me31B Fly)
   

   Tags

   Myc

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VP-hAR1-660

   Plasmid

   #89081

   Purpose

   mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids linked to VP16 activation domain

   Depositor

   Elizabeth Wilson

   Article

   Langley et al J Biol Chem. 1998 Jan 2;273(1):92-101.

   Insert

   hAR-1-660 (AR Human)
   

   Tags

   VP16

   Expression

   Mammalian

   Mutation

   aa 1-660 (contains exons A,B,C and part of D)

   Promoter

   SV40

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

   Plasmid

   #23000

   Depositor

   Jing Huang

   Article

   Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

   Insert

   mTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2035T aa 1968-2549 only

   Available Since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

   Plasmid

   #79914

   Purpose

   piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-CCHD (p130Cas)

   Plasmid

   #145132

   Purpose

   Expresses CCH domain from p130Cas with an EGFP tag

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   p130Cas CCH domain (BCAR1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_CRKL-2/2

   Plasmid

   #91364

   Purpose

   Protein expression and purification of human SH3 domain construct CRKL-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CRKL-2/2 (CRKL Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBp-FGFR2c-K660N

   Plasmid

   #45708

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR2 IIIc (FGFR2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K660N

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

10. p274 pCMV-CRE-M-AR(T)

    Plasmid

    #12497

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M AR (AR Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    Early termination mutation

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

11. C-His-GvpC-TEV

    Plasmid

    #153296

    Purpose

    Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    TEV-sensitive Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) 21 bp TEV recognition and cleavage site flanke…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BRCA1-(360-498)

    Plasmid

    #35068

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-360

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAM4663

    Plasmid

    #85126

    Purpose

    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiB-luciferase
    

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153540

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBp-FGFR2b-W290C

    Plasmid

    #45700

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    W290C

    Available Since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

16. BRCA1-(758-1057)

    Plasmid

    #35085

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-1057

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. lentiCRISPR v2-sgDnmt1_1

    Plasmid

    #138347

    Purpose

    Express guide RNA 1 for Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR v2-sgDnmt1_2

    Plasmid

    #138348

    Purpose

    Express guide RNA 2 for mouse Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-2XHA-Src-S4-UD-S32

    Plasmid

    #140302

    Purpose

    Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST40-2XHA-Src-S4-S32-KD

    Plasmid

    #140298

    Purpose

    Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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