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pCk2_spacer

Plasmid

#136072

Purpose

Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pH6HTC_minus_cer-region

Plasmid

#175297

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Type

Empty backbone

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-pHlu

Plasmid

#129315

Purpose

pH measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Promoter

ACT1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-CaaX(tH)

Plasmid

#109539

Purpose

Multisite gateway vector for 3' tagging with the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX domain from Hras

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-SGEF

Plasmid

#129620

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

SGEF

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG4

Plasmid

#129618

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG4

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FGD5

Plasmid

#129613

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I4R

Plasmid

#125066

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I4L

Plasmid

#125033

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_CoumRS

Plasmid

#64882

Purpose

Expresses Coumarinyl- tRNA synthetase

Depositor

Sebastián Brauchi

Article

Steinberg et al bioRxiv, 10.1101/065227

Insert

Coum_RS

Tags

Flag

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfCFPBb

Plasmid

#118232

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfCFP

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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EB3-3xmTq2-N1

Plasmid

#112929

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

3x mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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