We narrowed to 14,384 results for: nts

1. pcDNA5-FRT/TO-N-mEGFP-PARP7

   Plasmid

   #178004

   Purpose

   Expresses N-terminally mEGFP-tagged PARP7

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP7 (TIPARP Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. tdp43-EGFP construct6

   Plasmid

   #28199

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-314 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pEJS887_pTetR-P2A-BFPnls/sgAlpha

   Plasmid

   #108651

   Purpose

   Alpha satellite repeats targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Alpha satellite repeats targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Promoter

   U6 and hPGK

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Promoter

   Chicken ß-actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMX GFP Rac G12V

   Plasmid

   #14567

   Depositor

   Joan Brugge

   Article

   Hall et al Immunity. 2006 Mar . 24(3):305-16.

   Insert

   Rac1 G12V (RAC1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G12V

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdp43-EGFP construct7

   Plasmid

   #28200

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-274 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. CoV2-N-T205I

   Plasmid

   #177954

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (T205I) (N )
   

   Expression

   Mammalian

   Mutation

   T205I

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. VRQR variant

   Plasmid

   #138562

   Purpose

   Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_B2101

    Plasmid

    #91060

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKEE401

    Plasmid

    #91715

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC-HYG-YR

    Plasmid

    #61765

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-ITFG2

    Plasmid

    #87045

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. tdp43-EGFP construct8

    Plasmid

    #28201

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa275-313 deleted

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster nanos

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 DPP9S

    Plasmid

    #166789

    Purpose

    pDONR gateway shuttling vector with DPP9S (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Gateway shuttling vector

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EYFP-Mito-7

    Plasmid

    #56596

    Purpose

    Localization: Mitochondria, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-HLA-A1

    Plasmid

    #135503

    Purpose

    Mammalian expression of myc-tagged HLA-A*01:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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