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BASIC_6_J23102

Plasmid

#68140

Purpose

storage vector for BASIC part: J23102

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23102 Anderson Promoter

Expression

Bacterial

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#216098

Purpose

CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMA_Auxiliary 4B

Plasmid

#102713

Purpose

Provides the End-to-End linker 4B which assists Level 2 cloning in Mobius Assembly

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

Linker sequence

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1B

Plasmid

#102702

Purpose

Mobius Assembly Level 1 Acceptor Vector B

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA_Level 1Γ

Plasmid

#102703

Purpose

Mobius Assembly Level 1 Acceptor Vector Γ

Depositor

Naomi Nakayama

Article

Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

Insert

J23106:B0034:spisPink:rrnBT1-T7TE

Use

Synthetic Biology

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-C

Plasmid

#87540

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-G

Plasmid

#87541

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-T

Plasmid

#87534

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-A

Plasmid

#87535

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-C

Plasmid

#87536

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-G

Plasmid

#87537

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-T

Plasmid

#87538

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STAR-KRAB-A

Plasmid

#87539

Purpose

Destination vector for TALE-KRAB construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (repressor)

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1_H1-6

Plasmid

#223816

Purpose

Bacterial expression of Vipp1 H1-6 with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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sCatch-GFP-6xHIS

Plasmid

#186904

Purpose

Bacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

sfGFP-SpyCatcher003-sfCherry1-10-6xHIS

Tags

6xHis and sfGFP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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sTag-BFP-6xHIS

Plasmid

#186905

Purpose

Bacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-6xHIS

Tags

tagBFP, 6xHis

Expression

Bacterial

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pZS221

Plasmid

#177643

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K57A, K58A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS_m-AKAR5

Plasmid

#114139

Purpose

Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5 C-terminally tagged by KRas C tail

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1513-D4H*

Plasmid

#252026

Purpose

Safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging of the gene Vps32

Depositor

Thierry Soldati

Article

Perret et al Sci Adv. 2026 Jan 2;12(1):eady0812. doi:

Insert

D4H*

Use

Amoebal expression

Tags

GFP

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pPI419-D4H*

Plasmid

#252027

Purpose

Safe haven knock-in plasmid for the act5 locus, N-terminal mScarlet tagging

Depositor

Thierry Soldati

Article

Perret et al Sci Adv. 2026 Jan 2;12(1):eady0812. doi:

Insert

D4H*

Use

Amoebal expression

Tags

mScarlet

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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