We narrowed to 8,596 results for: Set

1. pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

   Plasmid

   #91912

   Purpose

   Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbnCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK234_027

   Plasmid

   #123907

   Purpose

   Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA1 -hThal5.10-2_hg18
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE673

   Plasmid

   #153239

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020

   Plasmid

   #91911

   Purpose

   Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbmCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC026 - PpCas13a from Paludibacter propionicigenes WB4

   Plasmid

   #91916

   Purpose

   Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   PpCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

   Plasmid

   #91914

   Purpose

   Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   CgCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK0_001

   Plasmid

   #123924

   Purpose

   Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   TU-sgRNA - L1/RE
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTK234_055

   Plasmid

   #123920

   Purpose

   Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCALNL_chr13_102010574-102010650

   Plasmid

   #81213

   Purpose

   pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Expression

   Mammalian

   Promoter

   pCBa

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL_Ch12_62418577-62418652

    Plasmid

    #81212

    Purpose

    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Expression

    Mammalian

    Promoter

    pCBa

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC215

    Plasmid

    #118879

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    

    Tags

    Dendra2, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC207

    Plasmid

    #118869

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mCherry

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLaNGo11-3

    Plasmid

    #126054

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMiNGo11-1

    Plasmid

    #126056

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)MBII-85-3'ss mut

    Plasmid

    #67644

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    3' splice site muattion

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC110

    Plasmid

    #118905

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and LPETGg

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC219

    Plasmid

    #118908

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    

    Tags

    YBBR tag, HIS10, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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