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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Publication

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Tags

C-Tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Publication

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSin Helper

Plasmid

#127694

Purpose

Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Publication

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Glycoproteins

Use

Synthetic Biology

Available since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Publication

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Publication

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFRT_TO_eGFP_TIA1

Plasmid

#106094

Purpose

gene expression of EGFP-TIA1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TIA1 (TIA1 Human)

Tags

EGFP

Expression

Mammalian

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-hERalpha

Plasmid

#101141

Purpose

Mammalian expression of full-length human estrogen receptor-alpha

Depositor

Elizabeth Wilson

Publication

Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

estrogen receptor-alpha (ESR1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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