164,799 results

1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Use

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcs2-GFP-IRES-mCherry

   Plasmid

   #226098

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CAG-dCasRx-RBM25-IRES-BleoR

   Plasmid

   #221002

   Purpose

   Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25-IRES-BleoR
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCre-P2A-mCherry

   Plasmid

   #212105

   Purpose

   Expression of Cre and mCherry in mammalian cells

   Depositor

   Daekee Lee

   Insert

   Cre recombinase and mCherry
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-mCherry

   Plasmid

   #20972

   Purpose

   H2B fused to mCherry for chromatin visualization

   Depositor

   Robert Benezra

   Article

   Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

   Insert

   Histone cluster 1, H2bb (H2BC3 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-SP6

   Plasmid

   #102418

   Purpose

   Ac transposase with SP6 promoter for in vitro transcription

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   Ac transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP6

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLX-PGK-hTERT

   Plasmid

   #114315

   Purpose

   Constitutive expression of the hTert protein

   Depositor

   Patrick Salmon

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-shRNA-ctrl

   Plasmid

   #85741

   Purpose

   shRNA ctrl

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   shRNA ctrl
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available Since

   April 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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