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Khc73(1-432)-GFP

Plasmid

#31748

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-432

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-9-sGPR124-VEGFR2 TMD-FKBP

Plasmid

#246697

Purpose

Expression vector for human GPR124 ECD fused to human VEGFR2 TMD and human FKBP1A F36V, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Publication

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and hVEGFR2 TMD-hFKBP1A F36V

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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TAPBPR S104F K211L R270Q

Plasmid

#245834

Purpose

expresses TAPBPR-S104F-K211L-R270Q in S2 cells

Depositor

Nikolaos Sgourakis

Publication

Sun et al Sci Adv. 2023 Feb 24;9(8):eade7151. doi:

Insert

TAPBPR (S104F K211L R270Q) (TAPBPL Human)

Tags

AviTag biotinylation tag and His6 affinity tag

Expression

Insect

Mutation

S104F K211L R270Q (HiFi mutants) & C94S (remo…

Promoter

MT_puro

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-NR5A1-deltaLBD-FLAG

Plasmid

#242228

Purpose

Expression of human SF-1 ∆LBD (deletion of a.a. 221-459) using piggyBac transposition

Depositor

Courtney Hodges

Publication

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Mutation

Deleted ligand binding domain (LBD) from SF-1 (de…

Promoter

CAG

Available since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-PA-BAX(ER, G108V)

Plasmid

#233344

Purpose

Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

BAX(3-171), N-terminus and C-terminus are deleted…

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K197R-VA

Plasmid

#191223

Purpose

Lentiviral expression of human IFIT5_K197R in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 197 to Arginine (K197R)

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-IFIT5_K160R-VA

Plasmid

#191222

Purpose

Lentiviral expression of human IFIT5_K160R in mammalian cells

Depositor

Mohan Babu

Publication

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

interferon-induced protein with tetratricopeptide repeats 5 (IFIT5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

changed Lysine 160 to Arginine (K160R)

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

Plasmid

#234737

Purpose

All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

Depositor

Kirill Martemyanov

Publication

Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

Insert

Nrl (Nrl Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hTyro_gR1

Plasmid

#225995

Purpose

Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Publication

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hAxl_gR1

Plasmid

#225994

Purpose

Expresses a chimeric contruct having N-terminal human Axl and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Publication

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human Axl N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (AXL, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* full-length

Plasmid

#221402

Purpose

Mammalian expression of human integrin beta1 Q280* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 K418* full-length

Plasmid

#221403

Purpose

Mammalian expression of human integrin beta1 K418* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* full-length

Plasmid

#221404

Purpose

Mammalian expression of human integrin beta1 N391* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N562* full-length

Plasmid

#221405

Purpose

Mammalian expression of human integrin beta1 N562* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCI AscI MR1 res

Plasmid

#214752

Purpose

expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

Depositor

David Lewinsohn

Publication

Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

Insert

MHC class I-related protein 1 (MR1 Human)

Tags

IRES eGFP

Expression

Mammalian

Mutation

silent mutations to prevent editing by CRISPR/Cas…

Promoter

CMV

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFBOH-MHL_WDR91:392-747_delta:522-536

Plasmid

#210881

Purpose

Insect expression of human WDR91 fragment P392 to A747 with loop deletion Q522 to N536. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747_delta:522-536 (WDR91 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

loop deletion amino acids Q522 to N536

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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