Addgene

We narrowed to 21,474 results for: ESC;

Showing: 10741 - 10770 of 21474 results

Results per page:

Sort results by:

lentiCRISPR-v2-HygR-EGFP

Plasmid

#167188

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and EGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Golgi-7

Plasmid

#58018

Purpose

Localization: Golgi Complex, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCerulean3-BclXL-pEGFP-C1

Plasmid

#177408

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pdRBFC-NS1-YN

Plasmid

#74723

Purpose

Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-LgBiT-2A-EGFP-Vin-PILATeS

Plasmid

#216761

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS

Use

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-i_alphaTubulin_C1

Plasmid

#85047

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX2-mCherry-CHMP2B

Plasmid

#115331

Purpose

expresses mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4205 C to T

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Tags

Expression

Mutation

S383T, F177A

Promoter

hSyn

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Tags

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pdRBFC-NS1-YC

Plasmid

#74724

Purpose

Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

Depositor

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

FLAG and YFP C-terminal half domain

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe(puro) mouse Egr2

Plasmid

#108097

Purpose

Retroviral vector for expression of mouse Egr2

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr2 (Egr2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Gamma-Tubulin-17

Plasmid

#58017

Purpose

Localization: Centrosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CVS-N2c-nl.mCherry-GCaMP7s

Plasmid

#172385

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized mCherry + GCaMP7s

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Vimentin-7

Plasmid

#58029

Purpose

Localization: Intermediate Filaments, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

NM_003380.3 (VIM Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Tags

Expression

Mutation

S383T, F177A

Promoter

hSyn

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ALIX(1-702)-mNeonGreen

Plasmid

#191193

Purpose

Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (1-702) (PDCD6IP Synthetic, Human)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Deletion of ALIX C-terminal PRD.

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Golgi-ExRai-AktAR2

Plasmid

#184051

Purpose

Golgi-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Golgi-ExRai-AktAR2

Use

Tags

N-terminal 35 amino acids from human endothelial …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Tags

Expression

Mutation

S383T

Promoter

EFS

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEZT-GST-SLFN2

Plasmid

#174319

Purpose

Expression of recombinant GST-SLFN2

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GST-SLFN2 (Slfn2 Mouse)

Use

Bacmam, baculovirus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FRT-EF1a-TVA-2A-N2cG

Plasmid

#172361

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-i_H2A_C1

Plasmid

#85053

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-deLACCO1-NGR

Plasmid

#208024

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCarCBADE_ProB(I69E)A

Plasmid

#117756

Purpose

ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac(UV5)

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge