We narrowed to 1,759 results for: plasmid for e coli
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Plasmid#188255PurposeIntegrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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pHG5
Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyExpressionBacterialMutationE59G - please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEL2200
Plasmid#170407PurposeGenetic complementation plasmid of LetA-[LetB PLL6->3] mutantDepositorExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 347-360)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2201
Plasmid#170408PurposeGenetic complementation plasmid of LetA-[LetB PLL6->5] mutantDepositorExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 580-600)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2254
Plasmid#170409PurposeGenetic complementation plasmid of LetA-[LetB PLL6->2] mutantDepositorExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 225-249)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2255
Plasmid#170410PurposeGenetic complementation plasmid of LetA-[LetB PLL6->4] mutantDepositorExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 445-479)Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only