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Plasmid#127778PurposeChicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)DepositorInsertsox3gRNAT2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1/MKRN1
Plasmid#78757PurposeTo purify GST-tagged MKRN1 recombinant proteinDepositorInsertMKRN1 (MKRN1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1 110-482
Plasmid#78747PurposeTo overexpress MKRN1 110-482 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutation110-482PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1 1-280
Plasmid#78750PurposeTo overexpress -MKRN1 1-280 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutation1-280PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1 N297A
Plasmid#66723PurposeConstitutive expression of FPR1 N297A in mammalian cells.DepositorInsertFPR1 (haplotype 3) (FPR1 Human)
UseTagsExpressionMammalianMutationN297APromoterSR-alphaAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S334A
Plasmid#51593Purposeexpresses RNAiR myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 334 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC:FCP:ShBle:FCP:EGFP
Plasmid#85987PurposeExpresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.DepositorInsertsShBle
EGFP
UseTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pSIV-D3psi/delta env/delta Vif/delta Vpr
Plasmid#132928PurposeProduction of SIV-VLPs containing Vpx proteinDepositorInsertGag, pol, and vpx
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C-GFP
Plasmid#195154Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistantPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-mitoLbNOX-FLAG
Plasmid#218667PurposepLVX-TetOne-Puro-mitoLbNOX-FLAGDepositorInsertmitoLbNOX
UseLentiviral and Synthetic BiologyTagsFLAG and MTSExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-LbNOX-FLAG
Plasmid#218631PurposeThis plasmid contains LbNOX under doxycycline inducible control.DepositorInsertLbNOX
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Beclin1
Plasmid#99574PurposeExpressed Cas9 with sgRNA targeting Beclin1DepositorInsertgRNA targeting Beclin1 (BECN1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast coUGP2
Plasmid#211531PurposeExpresses codon optimized UGP2DepositorInsertUGP2 (UGP2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9-mRuby2
Plasmid#183872PurposepX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.DepositorTypeEmpty backboneUseCRISPRTagsHypaCas9-P2A-mRuby2-T2A-PuroExpressionMammalianMutationPromoterCbhAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-c/o NL4-3 Env delta CT
Plasmid#139285PurposeHIV-1 NL4-3 glycoprotein lacking cytoplasmic tailDepositorInsertcodon optimized HIV-1 Env delta cytoplasmic tail
UseTagsExpressionMammalianMutationdeletion of cytoplasmic tail after residue 710PromoterCMV IEAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5M-hPR-B
Plasmid#89114Purposemammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinkerDepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutation5 restriction sites deleted in polylinkerPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mCherry
Plasmid#137794PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1.ARRDC1
Plasmid#38320DepositorInsertARRDC1 (ARRDC1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-AAVS1_sgRNA
Plasmid#183890PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.DepositorInsertAAVS1 sgRNA spacer (AAVS1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-PSM-Rb
Plasmid#37106DepositorInsertRb (RB1 Human)
UseRetroviralTagsExpressionMammalianMutationaa 379-928 only; large pocket of human Rb with aa…PromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mVenus
Plasmid#137793PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-RnBarr2
Plasmid#137800PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57mini ZM249M P90A _env eGFP
Plasmid#132939PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequenceDepositorInsertHIV-1ZM249M provirus (P90A) with env deletion, GFP
UseLentiviralTagsExpressionMutationP90APromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG-V5-Snail2-IRES-nls-GFP
Plasmid#44282DepositorInsertV5-Snail2 (SNAI2 Chicken)
UseTagsV5ExpressionMammalianMutationPromoterCMV-IE-enhancer and chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL652
Plasmid#159456PurposeDonor empty vector with puromycin resistance gene expression cassette flanked by FRTDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only