Addgene

We narrowed to 14,516 results for: pET

Showing: 701 - 720 of 14516 results

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pDRF1-GW ymYPET

Plasmid

#118455

Purpose

Overexpression of ymYPET

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Use

Tags

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-clbO

Plasmid

#114153

Purpose

Expresses ClbO

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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RalGDS-YPet

Plasmid

#134929

Purpose

Fluorescently tagged affinity reagent

Depositor

Klaus Hahn

Article

O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

Insert

RaIGDS RBD, YPet

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pET26b_AT118-L-V5-His

Plasmid

#222848

Purpose

E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

V5-His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETM11-ACS

Plasmid

#108943

Purpose

heterologous expression of ACS of E. coli in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Acetyl-CoA synthetase (ACS)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbK

Plasmid

#114152

Purpose

Expresses ClbK

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbK

Use

Tags

6xhis-Thrombin

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbI

Plasmid

#114151

Purpose

Expresses ClbI

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbI

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbF

Plasmid

#114148

Purpose

Expresses ClbF-cHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

ClbF

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbC

Plasmid

#114145

Purpose

Expresses ClbC-nHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbC

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbE

Plasmid

#114147

Purpose

Expresses ClbE-cHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

ClbE

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbD

Plasmid

#114146

Purpose

Expresses ClbD-cHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

ClbD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbG

Plasmid

#114150

Purpose

Expresses ClbG

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbBpks

Plasmid

#114154

Purpose

Expresses the PKS module of ClbB

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbB

Use

Tags

6xHis

Expression

Bacterial

Mutation

Only contains the PKS module of ClbB

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4CyPet-MAMM

Plasmid

#14033

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Insert

CyPet

Use

Tags

Expression

Mammalian

Mutation

Mammalian optimized CyPet

Promoter

Available since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET22HT-PP7delFG

Plasmid

#31863

Depositor

Robert Singer

Article

Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

Insert

PP7delFG Coat Protein

Use

Tags

His6-tag and TEV site

Expression

Mutation

deleted FG loop

Promoter

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

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