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pcDNA3.1(+)-C-6His-mEsrra

Plasmid

#173152

Purpose

mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

Depositor

Brijesh Kumar Singh

Article

Tripathi et al bioRxiv 2021.11.16.468891

Insert

ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)

Use

Tags

His

Expression

Mammalian

Mutation

Wild type

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_AAVS1_1801

Plasmid

#159090

Purpose

targeting, non-essential locus control

Depositor

Rory Johnson

Article

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Insert

gRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCG_SARS-CoV1 NSP1 C-V5-tag

Plasmid

#179961

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp1 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND3-Puro

Plasmid

#172622

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP15 C-V5-tag

Plasmid

#179984

Purpose

Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 Nsp15

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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VN-PTEN

Plasmid

#89331

Purpose

expresses hPTEN fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

Insert

PTEN1 (PTEN Human)

Use

Tags

N-terminal part of Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND2-Puro

Plasmid

#172628

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A- L325E

Plasmid

#166464

Purpose

Expresses fusion of mEGFP and YAPS127A- L325E

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A- L325E (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

YAPS127A- L325E

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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sgAPC(B)

Plasmid

#85558

Purpose

Expresses sgRNA targeting human APC around codon I1417

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

APC, WNT signaling pathway regulator (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
B*07:05:01

Plasmid

#203268

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*07:05:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-2xFLAG-2xSTREP-CCND1(P287S)-IRES-mCherry

Plasmid

#172635

Purpose

Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287S) and mCherry in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Ser

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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A*68:01:01

Plasmid

#203292

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*68:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup-deltaC

Plasmid

#90023

Purpose

To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Deletion of C-terminal region

Promoter

CMV

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-CAGGS-ERAS

Plasmid

#52416

Purpose

Lentiviral vector encoding constitutive expression of human ERAS cDNA

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

Plasmid

#170196

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Use

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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