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We narrowed to 150,556 results for: ins

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Showing: 7301 - 7320 of 150556 results

  5. pMAL-Abl-PRM 4R

    Plasmid
    #112087
    Purpose
    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
    Depositor
    Michael Rosen
    Article
    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi
    Insert
    Abl-PRM-4R (ABL1 Human)
    Tags
    His-6 and MBP
    Expression
    Bacterial
    Mutation
    construct contains only the PRM motif of Abl1
    Promoter
    Lac
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112087-plasmid-map-sequence-id-236084

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  16. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  18. TDP-REGv2 Cryptic PE-Max

    Plasmid
    #216163
    Purpose
    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    PEMax for prime editing with internal cryptic exon
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216163-plasmid-map-sequence-id-437751

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Showing: 7301 - 7320 of 150556 results