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SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12 (S SARS-CoV-2)

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-GFPenhancer

Plasmid

#202468

Purpose

Expresses the nanobody GFP enhancer after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP enhancer

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-GFPminimizer

Plasmid

#202469

Purpose

Expresses the nanobody GFP minimizer after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAP007

Plasmid

#206809

Purpose

pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

Depositor

Daan Swarts

Article

Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

Insert

BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm1

Plasmid

#222911

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm4

Plasmid

#222914

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm3

Plasmid

#222913

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm2

Plasmid

#222912

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pNK6031

Plasmid

#219739

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCV

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

Cestrum yellow leaf curling virus (CmYLCV) promoter

Use

Synthetic Biology

Expression

Plant

Promoter

CmYLCV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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