We narrowed to 9,391 results for: BLI

1. pCAGGS-FLAG-BubR1-D911A

   Plasmid

   #200815

   Purpose

   expresses BubR1-D911A in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Aspartic acid 911 to Alanine

   Promoter

   CAG

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE

   Plasmid

   #231804

   Purpose

   Label centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.

   Depositor

   Annette Oxenius

   Insert

   Kaede-CETN1 (Cetn1 Mouse)
   

   Use

   Lentiviral

   Tags

   Kaede

   Expression

   Mammalian

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

   Plasmid

   #225438

   Purpose

   Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

   Depositor

   Diego Orzaez

   Insert

   CBS-FRT-OCS-BeYDVRepRepA-tNos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

   Plasmid

   #225436

   Purpose

   BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

   Depositor

   Diego Orzaez

   Insert

   BeYDV geminino 1.0
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

   Plasmid

   #225430

   Purpose

   OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

   Depositor

   Diego Orzaez

   Insert

   miniDFR-FRT-OCSterm-FRT-miniDFR
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-UBE3A iso 2 N terminus

   Plasmid

   #185643

   Purpose

   expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells

   Depositor

   Irina Bezsonova

   Insert

   ubiquitin-protein ligase E3A (UBE3A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEC2796 (pSpy0C4)

   Plasmid

   #220280

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN993

   Plasmid

   #219961

   Purpose

   Expresses CMT2A mutant version of human KIF1Bß in C. elegans

   Depositor

   Shinsuke Niwa

   Insert

   unc-104 promoter::human KIF1Bß(Q98L) (KIF1B Human, Nematode)
   

   Expression

   Worm

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSM045

    Plasmid

    #217399

    Purpose

    KmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

    Depositor

    Brian Pfleger

    Article

    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

    Insert

    BTE1
    

    Expression

    Bacterial

    Promoter

    PTrc

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC-C-SF-TAP-DEST-TLK1- M566T

    Plasmid

    #218831

    Purpose

    Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

    Depositor

    Travis Stracker

    Insert

    TLK1 M566T (TLK1 Human)
    

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    M566T

    Promoter

    CMV

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG426-GAL-ATXN1-Q85

    Plasmid

    #181712

    Purpose

    Galactose inducible expression of ATXN1-Q85

    Depositor

    Alejandro Chavez

    Insert

    ATXN1-Q85 (ATXN1 Human)
    

    Expression

    Yeast

    Mutation

    extended repeat of 85 Gln residues replacing nati…

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H

    Plasmid

    #203901

    Purpose

    Reporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.

    Depositor

    Carolyn Lee-Parsons

    Insert

    pD4H-FLUC-tOCS; pNOS-RLUC-tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSM046

    Plasmid

    #217400

    Purpose

    KmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under Ptrc

    Depositor

    Brian Pfleger

    Article

    Mishra et al J Ind Microbiol Biotechnol. 2024 Feb 16:

    Insert

    BTE2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PTrc

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #219653

    Purpose

    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

    Depositor

    Thomas Oertner

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

    Expression

    Mammalian

    Promoter

    nEf Addgene # 149296

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid

    #208625

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 72~197, other residues not included

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PE-SUMO CRYBA4

    Plasmid

    #217671

    Purpose

    For expression of SUMO tagged human beta A4 crystallin

    Depositor

    Larry David

    Insert

    CRYBA4 (CRYBA4 Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG416-GAL-FUS-R521C

    Plasmid

    #181718

    Purpose

    Galactose inducible expression of FUS-R521C

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    R521C

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG416-GAL-FUS-P431L

    Plasmid

    #181717

    Purpose

    Galactose inducible expression of FUS-P431L

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    P431L

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CTNNB1 - G752A in pENTR

    Plasmid

    #216623

    Purpose

    gateway cloning donor vector containing CTNNB1 ASD mutation

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Gateway entry plasmid

    Mutation

    G752A

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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