We narrowed to 15,732 results for: LEA

1. TVBB C-term-mScarlet-Puro

   Plasmid

   #169220

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MMLV-gag-hMLH1dn

   Plasmid

   #195513

   Purpose

   MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn (MLH1 Human)
   

   Use

   Virus like particle

   Tags

   FLAG, NES, and Protease cleavate site

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   CMV

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. hTph2p-Luc

   Plasmid

   #223526

   Purpose

   Firefly-luciferase reporter expression driven by human Tph2 promoter

   Depositor

   Kyungjin Kim

   Article

   Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

   Insert

   Tph2 promoter (TPH2 Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Promoter

   hTph2 promoter

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TVBB C-term-mNeongreen-Puro

   Plasmid

   #169228

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

   Plasmid

   #181744

   Purpose

   bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY-HF1
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

   Promoter

   T7

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2-CAG-3xNLS-AausFP1

   Plasmid

   #191096

   Purpose

   To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-AausFP1
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-flag-hKDM4A(H188A)-polyA

   Plasmid

   #101052

   Purpose

   in vitro transcription (T7 promoter)

   Depositor

   Yi Zhang

   Article

   Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

   Insert

   KDM4A (KDM4A Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Sept. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-GRAB_CRF1.0

   Plasmid

   #208661

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pelB-MBP-BB0323-ss

    Plasmid

    #137067

    Purpose

    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66700.1
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature BB0323: lacks the BB0323 signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCWXPGR-pTF-dnTCF4

    Plasmid

    #114277

    Purpose

    TET-inducible expression of a dominant negative form of the TCF4 transcription factor

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Mutation

    deletion of AA 1 to 31

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKO_intergenic-intergenic_2

    Plasmid

    #155078

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC589 - pAAV c-fos Nuc-eYFP

    Plasmid

    #59133

    Purpose

    An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    mFos

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2443

    Plasmid

    #72252

    Purpose

    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3.7-EF-EYFP-YAP1_S94A-PolyA

    Plasmid

    #112286

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with Serine 94 to Alanine

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-shMcu-1

    Plasmid

    #181868

    Purpose

    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

    Insert

    mitochondrial calcium uniporter (Mcu Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    hrGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-myc-ERK2-MEK1_fusion

    Plasmid

    #39194

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

19. RCAS-Flag-BRAFV600E

    Plasmid

    #126615

    Purpose

    Retroviral expression of Flag tagged human oncogenic BRAFV600E

    Depositor

    Yi Li

    Article

    Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

    Insert

    BRAF (BRAF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V600E

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY0738 pCAG-NLS-SscINT

    Plasmid

    #179113

    Purpose

    SscINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SscINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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