We narrowed to 8,648 results for: FIE

Showing: 7341 - 7360 of 8648 results

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pBR BsmAIM Kan + p15A BsaIR Amp

Plasmid

#206208

Purpose

Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

BsamAIM and BsaIR

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBK-CMV-hNHERF2

Plasmid

#47801

Purpose

Expresses human NHERF2 in mammalian cells

Depositor

Randy Hall

Article

Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

Insert

NHERF2 (NHERF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGGAselect

Plasmid

#195714

Purpose

pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pXJ-Myc-IRF2

Plasmid

#232642

Purpose

transient expresses IRF2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF2 (IRF2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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M13-kmR

Plasmid

#206861

Purpose

M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

Depositor

Irene Chen

Article

Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

Insert

Kanamycin resistance gene

Expression

Bacterial

Promoter

lac

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFPC1-hMyoX

Plasmid

#47608

Depositor

Emanuel Strehler

Article

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

MyoX (MYO10 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pPE3-pPuro

Plasmid

#215722

Purpose

Cloning vector for Uni-Vector prime editing system

Depositor

Kou-Juey Wu

Article

Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

Insert

PE2

Use

Unspecified

Promoter

EFS promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Dat - EGFP - Tol2

Plasmid

#85418

Purpose

for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

Depositor

Marc Ekker

Article

Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

Insert

dopamine transporter (slc6a3 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Promoter

dat

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only
pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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CROPseq-tRFP

Plasmid

#230938

Purpose

Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

Depositor

Hyejung Won

Article

Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pCSF107mT-GATEWAY-3'-FLAG

Plasmid

#67619

Purpose

A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Flag

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA C/EBP gamma

Plasmid

#15715

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

C/EBP gamma (CEBPG Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pNCS mStrawberry

Plasmid

#91768

Purpose

Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Strawberry

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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