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CROP_C9_Puro_NTC4

Plasmid

#183336

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC4

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC3

Plasmid

#183335

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC3

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC1

Plasmid

#183333

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC1

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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NSK163 CMV-TO-SMN2 Nluc - active (exon 6, 7, 8)

Plasmid

#247276

Purpose

Expresses human SMN2 exons 6, 7, 8 followed by Nluc

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SMN2 exons 6,7,8-Nluc (SMN2 Human)

Expression

Mammalian

Mutation

"A" insertion at position 49 of exon 7

Promoter

CMV-TO

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TO-CasRx(NLS)-T2A-mCherry

Plasmid

#242433

Purpose

CasRx (NLS-RfxCas13d-NLS) for RNA targeting under the CMV-TO promoter (CMV with two Tet-Operators)

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

CasRx

Use

CRISPR

Tags

Tag / Fusion Protein: T2A mCherry

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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NSK477 SFFV-mCherry-SGc(Smn2ex7.os13-72bp.lowBG.16bp loop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247291

Purpose

Low baseline modulADAR sensor for SMN exon 7

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-Smn2ex7.os13-72bp.lowBG.16bp loop-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK465 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247290

Purpose

Low baseline modulADAR sensor for NOT SCN1a exon 20N

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-os13.NOT-SCN1A_20N.45,64,45.lowBG.12bp loop-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK418 SFFV-mCherry-SGc(s70587.os13.lowbBG.16bp loop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247289

Purpose

Low baseline modulADAR sensor for synthetic target transcript

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-os13.s70587.os13.lowbBG.16bp loop-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK249 CMV-TO-SCN1A (exons 20, 21)

Plasmid

#247284

Purpose

Expresses human SCN1a exons 20, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

SCN1a exons 20, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Gai-mTagBFP-3BEY-P2A-Gid-4LTB-GFP

Plasmid

#240137

Purpose

Mammalian plasmid expression both components of synDrops separated using P2A

Depositor

Liam Holt

Article

Shu et al PRX Life. 2024 Sep;2(3):033001. doi: 10.

Inserts

Gai-mTagBFP-3BEY
Gid-4LTB-GFP

Expression

Bacterial and Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCA2)

Plasmid

#235069

Purpose

Expresses Tad-CBE(CCA2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCA2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT1)

Plasmid

#235076

Purpose

Expresses Tad-CBE(GCT1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCT2)

Plasmid

#235077

Purpose

Expresses Tad-CBE(GCT2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCT2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG1)

Plasmid

#235079

Purpose

Expresses Tad-CBE(GCG1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACG1)

Plasmid

#235063

Purpose

Expresses Tad-CBE(ACG1) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACG1)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CT619

Plasmid

#233677

Purpose

Red light-inducible Cre-N; Cre-N270-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N270-PhyA

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT366

Plasmid

#233678

Purpose

Red light-inducible Cre-N; Cre-N105-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N105-FHY1

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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