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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Article

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Hygro-EF1A>{H2B-RFP}

Plasmid

#249059

Purpose

Nuclear RFP labeling

Depositor

Ori Bar-Nur

Article

Gjonlleshaj et al Cell Rep Methods. 2026 Feb 23;6(2):10129

Insert

H2B

Use

Lentiviral

Tags

mRFP1

Promoter

EF1a

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry

Plasmid

#248111

Purpose

Expresses dox-inducible ChemoCas13d or CasRx-QK634-LightR (UnirapR domain inserted right after amino acid residue 634 of CasRx)

Depositor

Jared Toettcher

Article

Zhu et al bioRxiv 2025.04.21.649818

Insert

CasRx-UnirapR

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

Tet inducible

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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LNPL503-piggybac-Dox-inducible-CasRx-QK634-LightR-IRES-mCherry

Plasmid

#248105

Purpose

Expresses dox-inducible OptoCas13d or CasRx-QK634-LightR (LightR domain inserted right after amino acid residue 634 of CasRx)

Depositor

Jared Toettcher

Article

Zhu et al bioRxiv 2025.04.21.649818

Insert

CasRx-LightR

Use

CRISPR and Synthetic Biology

Promoter

Tet inducible

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHD Btl-pYtag(Scarlet) ScarlessDsRed

Plasmid

#244937

Purpose

Vector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

Endogenous tagging

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAMKII-DrBphP-PCM

Plasmid

#241775

Purpose

To produce AAV9 encoding CAMKII-DrBphP-PCM

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

DrBphP-PCM

Use

AAV

Promoter

CAMKII

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mem-seTurboID

Plasmid

#237888

Purpose

AAV vector encoding membrane-tethered seTurboID under the control of human synapsin promoter

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

mem-seTurboID

Use

AAV

Tags

GAP43 palmitoylation signal and V5 tag

Expression

Mammalian

Promoter

human Synapsin 1 (hSyn)

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-seTurboID-IRES-tTA

Plasmid

#240206

Purpose

Amplifier AAV vector of Dual-AAV sparse labeling system encoding seTurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

seTurboID

Use

AAV

Tags

FLAG tag

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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iSM-IP

Plasmid

#235990

Purpose

Expresses iSM (iSpyMac) in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEPHS1CB0027

Plasmid

#227570

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_TkTXSS (T374G/E379D/Q486H/A745P)_35S

Expression

Plant

Mutation

T374G / E379D / Q486H / A745P, BsaI sites removed…

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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M6667

Plasmid

#219772

Purpose

MoClo-compatible Level 0 vector carrying terminator ORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Terminator ORCA3

Use

Synthetic Biology

Expression

Plant

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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M6570

Plasmid

#219773

Purpose

MoClo-compatible Level 0 vector carrying promoter pORCA3

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Promoter ORCA3

Use

Synthetic Biology

Expression

Plant

Promoter

pORCA3

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pNK3625

Plasmid

#219770

Purpose

MoClo-compatible Level 0 vector carrying promoter pWRKY70

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

Promoter WRKY70

Use

Synthetic Biology

Expression

Plant

Promoter

pWRKY70

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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