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pBEL2251

Plasmid

#170403

Purpose

For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2252

Plasmid

#170404

Purpose

For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 455-479)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2253

Plasmid

#170405

Purpose

For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Use

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB200

Plasmid

#169111

Purpose

TESEC backbone for target expression

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

lacZ (lacZ Escherichia coli K12 MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mCherry (KmR)

Plasmid

#177207

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Synthetic constitutive promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EcMscL

Plasmid

#92418

Purpose

E. coli Mechanosensitive Channel of Large Conductance

Depositor

Douglas Rees

Insert

MscL (mscL E. coli)

Use

Tags

6x Histidine Tag and Thrombin Cleavage Site betwe…

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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p416-GPD-HA-PPK

Plasmid

#183943

Purpose

Expresses HA-tagged E. coli PPK from yeast GPD promoter

Depositor

Michael Downey

Insert

PPK (ppk E. coli)

Use

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mCherry (ErmR)

Plasmid

#177206

Purpose

Plasmid with p15A origin for constitutive mCherry expression in E. coli

Depositor

Alexey Merz

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Synthetic constitutive promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAIP4_1TJE

Plasmid

#234203

Purpose

Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli

Depositor

Christopher Bahl

Insert

1TJE

Use

Tags

10x HisTag-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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