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Showing: 361 - 370 of 433 results

  4. pGJJ045

    Plasmid
    #183621
    Purpose
    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183621-plasmid-map-sequence-id-361478

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  6. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  7. pUpstream2Lox

    Plasmid
    #164573
    Purpose
    Integration of 2 LoxN sites in the genome of P. berghei
    Depositor
    Olivier Silvie
    Inserts
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164573-plasmid-map-sequence-id-325424

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  8. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Miguel Valvano
    Article
    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep
    Insert
    Ω trimethoprim cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    130660-plasmid-map-sequence-id-258172

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  10. pAAH1

    Plasmid
    #41825
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7, TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41825-plasmid-map-sequence-id-56469

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Showing: 361 - 370 of 433 results