We narrowed to 1,843 results for: FAST

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pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

Plasmid

#83946

Purpose

Cas9-carrying vector with kanMX marker

Depositor

Irina Borodina

Article

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

Cas9

Expression

Yeast

Available Since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSems LifeAct-StayGold

Plasmid

#222947

Purpose

Expression of LifeAct tagged with StayGold in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

LifeAct

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mDLX-TVA-2A-N2cG

Plasmid

#172364

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Expression

Mammalian

Promoter

mDLX

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-mTurquoise

Plasmid

#172376

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

mTurquoise

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Article

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pJS167

Plasmid

#48881

Purpose

Positive feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Inserts

araC gene with ssrA tag
monomeric yeast-enhanced green fluorescent protein

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Promoter

Para/lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-PhoCl2f

Plasmid

#164036

Purpose

Improved PhoCl variant with faster dissociation rate

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2f

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-jGCaMP8m

Plasmid

#172388

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

GCaMP8m

Use

Neurotropic virus

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-N2c_envA-IRES-Neo

Plasmid

#172368

Purpose

Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

envA + Neo

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-SADB19_oG-IRES-Puro

Plasmid

#172367

Purpose

Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oG + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRU55

Plasmid

#167684

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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