We narrowed to 23,753 results for: Spr

1. pLentiCRISPR-v2 hygro sgUCK1

   Plasmid

   #211524

   Purpose

   Deletes UCK1

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgUCK1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAf-CRISPR-ctfR1

   Plasmid

   #207992

   Purpose

   CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   ctfR1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus flavus U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-bleo-ErbB4

   Plasmid

   #197353

   Purpose

   A knock-out vector for dog ErbB4.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRa_all-in-one_Bip gRNA1

   Plasmid

   #183696

   Purpose

   Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   gRNA targeting chinese hamster Bip (Hspa5)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-HISMX6-pSPR3

   Plasmid

   #191792

   Purpose

   Yeast gene induction with meiotic promoter

   Depositor

   Dean Dawson

   Insert

   HIS3 pSPR3
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-pSPR3

   Plasmid

   #191788

   Purpose

   Yeast gene induction with meiotic promoter

   Depositor

   Dean Dawson

   Insert

   KanMX pSPR3
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR2_+10D+10U

   Plasmid

   #149394

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted upstream and downstream of the IHFp…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-NF1-L1-dSpRY

   Plasmid

   #177681

   Purpose

   Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   FokI-dCas9-SpRY
   

   Use

   CRISPR; Gateway compatible foki-dcas9-spry entry …

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-NF1-L2-dSpRY

   Plasmid

   #177682

   Purpose

   Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   FokI-dCas9-SpRY
   

   Use

   CRISPR; Gateway compatible foki-dcas9-spry entry …

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2-bleo-dEgf

    Plasmid

    #173840

    Purpose

    A knock-out vector for the dog Egf

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-puro-dHbegf

    Plasmid

    #173841

    Purpose

    A knockout vector for the dog Hbegf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiCRISPRv2-neo-dTgfa

    Plasmid

    #173842

    Purpose

    A knockout vector for the dog Tgfa.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2-hyg2-dEreg

    Plasmid

    #173843

    Purpose

    A knockout vector for the dog Ereg.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2-puro-dEgfr

    Plasmid

    #173844

    Purpose

    A knockout vector for the dog Egfr.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUPD2 NtSPrbcS (GB2213)

    Plasmid

    #160589

    Purpose

    B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain

    Depositor

    Diego Orzaez

    Insert

    NtSPrbcS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p8116 LentiCRISPR v2 sgPTPN14-4

    Plasmid

    #163316

    Purpose

    Expresses Cas9 and a human PTPN14-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

    Insert

    sgPTPN14-4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p8115 LentiCRISPR v2 sgPTPN14-3

    Plasmid

    #163314

    Purpose

    Expresses Cas9 and a human PTPN14-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgPTPN14-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPRi-CENPB-g10

    Plasmid

    #120218

    Purpose

    CENPB CRISPRi guide RNA 10

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 SCAF4_CRISPR resistant

    Plasmid

    #122465

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 CRISPR resistant (SCAF4 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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