We narrowed to 44,092 results for: cha

1. pcDNA3-PPO-Venus

   Plasmid

   #139502

   Purpose

   Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EcMscS

   Plasmid

   #78555

   Purpose

   E. coli Mechanosensitive Channel of Small Conductance

   Depositor

   Douglas Rees

   Article

   Bass et al Science. 2002 Nov 22;298(5598):1582-7.

   Insert

   Mechanosensitive Channel of Small Conductance
   

   Use

   Tags

   6x Histidine Tag

   Expression

   Mutation

   Promoter

   T7 Promoter

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-DIO-PPO-Venus

   Plasmid

   #139504

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV5

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1 hPHGDH

   Plasmid

   #154916

   Purpose

   Doxycyclin inducible expression of human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST PHGDH

   Plasmid

   #154917

   Purpose

   Expresses human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-BLAST co hPHGDH

   Plasmid

   #154915

   Purpose

   Express codon optimized human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH, codon optimized (PHGDH Human, Synthetic)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNC1124

   Plasmid

   #41560

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9
   

   Use

   Tags

   Expression

   Mutation

   GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

   Promoter

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNC1136

    Plasmid

    #41562

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNC1137

    Plasmid

    #41563

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX6P1-C. thermophilum LIC

    Plasmid

    #74780

    Purpose

    encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    dynein light intermediate chain (Chaetomium thermophilum)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-gst-xr

    Plasmid

    #214761

    Purpose

    Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

    Depositor

    Pimchai Chaiyen

    Article

    Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

    Insert

    glutathione S-transferases fused with xylose reductase
    

    Use

    Tags

    Glutathione S-transferases

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7m-psRaf1

    Plasmid

    #89364

    Purpose

    A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psRaf1 (RAF1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLL3.7m-psCDK5

    Plasmid

    #89365

    Purpose

    A lenti-viral vector expressing photoswitchable CDK5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psCDK5 (CDK5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

    Plasmid

    #226884

    Purpose

    Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

    Depositor

    Ning Zheng

    Article

    Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

    Insert

    NaVAb
    

    Use

    Tags

    MBP

    Expression

    Insect

    Mutation

    R4A, N49K, L109A, M116V

    Promoter

    Polyhedrin

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP64-hERG1a S620T

    Plasmid

    #53059

    Purpose

    Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Serine 620 to Threonine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rabbit Cav 2.2 W391A pMT2

    Plasmid

    #58734

    Purpose

    expression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2

    Depositor

    Annette Dolphin

    Article

    Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

    Insert

    Cav2.2 (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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