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(Plasmid #126551)


Item Catalog # Description Quantity Price (USD)
Plasmid 126551 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    Addgene plasmid 18917
  • Backbone manufacturer
    Addgene plasmid 18917
  • Backbone size w/o insert (bp) 5045
  • Total vector size (bp) 7604
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher)
  • Growth instructions
    Grow at 30 degrees in 2xYT media (see comments section below for more information). Please use 20 ug/ml of Amp.
  • Copy number
    Low Copy


  • Gene/Insert name
    eGFP N-terminal fused to FMRFamide gate sodium channel
  • Alt name
  • Species
    Helix aspersa
  • Insert Size (bp)

Resource Information

Depositor Comments

To minimize recombination, propagate this plasmids in Stbl2 cells. Also, to minimize recombination, cells should be cultured at 30 C.

Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended.

For more information on growth conditions, please see as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. The expected patterns can be calculated from the attached sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-Flex-rev-GFPFANACfullWT was a gift from Rory McQuiston (Addgene plasmid # 126551 ; ; RRID:Addgene_126551)
  • For your References section:

    Heterologous expression of the invertebrate FMRFamide-gated sodium channel as a mechanism to selectively activate mammalian neurons. Schanuel SM, Bell KA, Henderson SC, McQuiston AR. Neuroscience. 2008 Aug 13;155(2):374-86. doi: 10.1016/j.neuroscience.2008.05.052. Epub 2008 Jun 10. 10.1016/j.neuroscience.2008.05.052 PubMed 18598740