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Plasmid#100097PurposeFRET-based ECFP-YPET reporter that is cleaved by TEVDepositorInsertECFP-TevS-YPET
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mito-ZapCY1
Plasmid#58996PurposeMitochondrial Zn-binding FRET biosensorDepositorInsertmito-ZapCY1 high affinity Zn(II) sensor
UseTagsSee commentExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRD
Plasmid#172931PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRE
Plasmid#172932PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRE
Plasmid#172926PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-Cb5-pEGFP-C1
Plasmid#166764PurposeTo target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.DepositorInsertCb5 tail anchor (Cyb5a Rat)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-MAO-pEGFP-C3
Plasmid#166765PurposeTo target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertMAO tail anchor (MAOA Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionKE
Plasmid#172925PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorKE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mito-ZifCV1.49
Plasmid#59009PurposeMitochondrial Zn-binding FRET biosensorDepositorInsertmito-ZifCV1.49 genetically encoded Zn(II) sensor
UseTagsSee commentExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mito-ZifCV1.173
Plasmid#59010PurposeMitochondrial Zn-binding FRET biosensorDepositorInsertmito-ZifCV1.173 genetically encoded Zn(II) sensor
UseTagsSee commentExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Shark-tSH2
Plasmid#126672PurposeExpression of Shark tandem SH2 domains SNAP tagged for use as FRET reporterDepositorInsertShark tandem SH2 domains
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H127
Plasmid#170343Purpose6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-EV-D
Plasmid#125200PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-EV-D
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_crGE
Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4
Plasmid#61619PurposeFRET-based biosensor for monitoring PKA activityDepositorInsertAKAR4
UseTagsCerulean and cpVenus[E172]ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
vgCam-pRSETb
Plasmid#193362PurposeA FRET-based calcium probe using SumireDepositorInsertvgCam
UseE.coli expressionTagsHis X6 tag and Strep tagExpressionMutationPromoterT7Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW AKAR3-EV
Plasmid#182533PurposeExpresses the PKA FRET sensor AKAR3-EV in budding yeastDepositorInsertAKAR3-EV
UseTagsExpressionYeastMutationPromoterPMA1Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLIParaF.Ec-250n
Plasmid#18855DepositorInsertarabinose FRET sensor
UseTagsCFP and VenusExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only