We narrowed to 12,873 results for: sequence

1. HaloTag-beta2-adrenergic receptor

   Plasmid

   #66994

   Purpose

   Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

   Depositor

   Catherine Berlot

   Insert

   HaloTag-beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   Human Growth hormone Signal Sequence-HaloTag

   Expression

   Mammalian

   Mutation

   The signal sequence from human growth hormone was…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. T777T

   Plasmid

   #113082

   Purpose

   Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERmoxGFP

   Plasmid

   #68072

   Purpose

   mammalian expression of ER localized moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   moxGFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-Tol2

   Plasmid

   #31831

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Lac

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPROK AdoHcyase

   Plasmid

   #14683

   Depositor

   Michael Hershfield

   Article

   Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

   Insert

   adenosyl-homocysteine hydrolase (AHCY Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

8. mNeonGreen-KDEL

   Plasmid

   #226568

   Purpose

   Encodes mNeonGreen with KDEL ER lumenal target sequence

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   KDEL (ER lumenal target sequence)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-At-TRL

   Plasmid

   #32242

   Depositor

   Jay Hesselberth, Stan Fields

   Article

   Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

   Insert

   A. thaliana tRNA ligase (RNL Mustard Weed)
   

   Use

   Tags

   6xHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA Polmyerase

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p212 pCMV-EGFP/RFP

    Plasmid

    #8389

    Purpose

    Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDYU1G/γ-tubulin-mEGFP

    Plasmid

    #154301

    Purpose

    Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    actin15

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Puro HA-Ubiquitin-K48

    Plasmid

    #236290

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Ubiquitin K48 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Single ubiquitin coding sequence containing K48 m…

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLenti Puro HA-Ubiquitin-K63

    Plasmid

    #236291

    Purpose

    Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Ubiquitin K63 (UBB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Single ubiquitin coding sequence containing K63 m…

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDYU1B/mRuby3–α-tubulin

    Plasmid

    #154302

    Purpose

    Expresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    alpha-tubuin
    

    Use

    Dictyostelium expression

    Tags

    mRuby3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-CBE6b-IVT

    Plasmid

    #215834

    Purpose

    Template for in vitro transcription of CBE6b (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pG108 - K

    Plasmid

    #178039

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Insert

    Kanamycin resistance
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ErmAM has been replaced with Kanamycin for select…

    Promoter

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIKOarsBCm

    Plasmid

    #46763

    Purpose

    E.coli genomic integration at arsB

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9-CBE6c-IVT

    Plasmid

    #215835

    Purpose

    Template for in vitro transcription of CBE6c (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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