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GlycophorinB-CD4d3+4-bio

Plasmid

#73094

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinB with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin B (GYPB Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-HA

Plasmid

#209237

Purpose

Mammalian expression of ATP11C

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJH3628

Plasmid

#191445

Purpose

Prgef-1-Chrimson::UrSL2::wCherry unc-54 3' UTR C.elegans panneural expression of Chrimson RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Chrimson

Tags

wCherry

Expression

Worm

Promoter

Pregf-1

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Neuroplastin-CD4d3+4-bio

Plasmid

#73111

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Neuroplastin (NPTN Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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TIA1 RRM-YFP-SspBx6

Plasmid

#103781

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-YFP-SspBx6

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pSUC2::GNSI (NrsR)

Plasmid

#121537

Purpose

This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.

Depositor

Alexey Merz

Article

Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

Inserts

CYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR

Tags

mGFP5

Expression

Yeast

Mutation

M109I (Naturally occurring SNP) and The first 21 …

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pmEos2-C1-centrin-1

Plasmid

#72588

Purpose

mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

Depositor

Michel Bornens

Article

Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

Insert

centrin 1 (CETN1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJB556

Plasmid

#115966

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/15 SGP 2xTetApt-STOP

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2523

Plasmid

#191358

Purpose

Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP3

Tags

mCherry

Expression

Worm

Promoter

Punc-25

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Gal4 PGC1 alpha 1-550

Plasmid

#8894

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-550 of PGC-1 alpha

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

Plasmid

#136513

Purpose

Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

Depositor

Erik Sontheimer

Article

Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

Insert

codon-optimized AcrIIA5

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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CD58-CD4d3+4-bio

Plasmid

#73113

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD58 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD58 (CD58 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG ID2 hygro

Plasmid

#98394

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Tags

6xHA

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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