We narrowed to 13,692 results for: Egf
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pBa.Lamp1-GFP
Plasmid#180250PurposeMammalian expression of LAMP1DepositorInsertLAMP1 (LAMP1 Human)
UseTagsExpressionMammalianMutationWTPromoterChicken Beta actinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WAVE 2
Plasmid#67098PurposeStandard for cell-free expression benchmarking.DepositorInsertWAVE 2 (WASF2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1-Lyn-mCitrine-EEVEE-LifeAct
Plasmid#155226PurposeAlternate MPAct using Lyn/LifeAct instead of CaaX/F-tractinDepositorInsertLyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-p53 (NES-)
Plasmid#12092DepositorInsertp53 (NES-) (TP53 Human)
UseTagsGFPExpressionMammalianMutationL348A L350A Localized to nucleus.PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP-W
Plasmid#159291PurposeLentiviral vector expressing GFP driven by human EF1a promoterDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman EF1a promoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
InterCatch-1xL
Plasmid#186907PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance geneDepositorInsertTfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
hOC-GFPtpz
Plasmid#110207PurposeExpresses GFP in mature bone cells.DepositorInserteGFPtopaz
UseTagsNoneExpressionMammalianMutationPromoterHuman osteocalcin (BGLAP)Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP
Plasmid#199356PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lck-FRB-ECFP(W66A)
Plasmid#67902PurposeExpresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)DepositorInsertLck-FRB-ECFP(W66A)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
mMaroon-RTK-mMaroon
Plasmid#84359PurposeFRET acceptor for RhoA donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-Rtk--mMaroon
UseTagsmMaroon flanking Rtk domain on N and C terminusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NmCherry-mAID
Plasmid#140601PurposeExpression of OsTIR1 and mCherry-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mKO2-2A-mTurquoise2
Plasmid#98838PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmKO2-2A-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorInsertAP180 (Snap91 Rat)
UseTagsFKBP N-terminal GFP C-terminalExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
SLC30A10-GFP
Plasmid#104381PurposeExpresses SLC30A10-GFP wild-type in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32476PurposeExpresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP markerDepositorInsertPSAM Y115F,L141F-5HT3 HC
UseTagsIRES-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-2xrGBD
Plasmid#176109PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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GFP-mROCK1-delta2
Plasmid#101290PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation1-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRB-ECFP(W66A)-Giantin
Plasmid#67903PurposeExpresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)DepositorInsertFRB-ECFP(W66A)-Giantin
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Pac
Plasmid#126957PurposePromoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM18
Plasmid#133631PurposeIntegration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.DepositorInsertp1k-NAB2-GFP (NAB2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SeeSaw Reporter (SSR1.0)
Plasmid#50392PurposeSSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.DepositorInsertSSR1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta1
Plasmid#101289PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorInsertRock1 (Rock1 Mouse)
UseTagsExpressionMammalianMutation1-375 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AV09_pDonor.EP.S1.TS
Plasmid#100297PurposeAAVS1-targeting expression plasmidDepositorInsertPuroR.T2A.EGFP expression unit
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-shTACC3
Plasmid#59355PurposeKnocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.DepositorInsertEGFP
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
px458 EQR
Plasmid#101731PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
UseTags3xFLAG-NLS and NLSExpressionMammalianMutationEQR (D1135E, R1335Q, and T1337R mutations in Cas9)PromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH3
Plasmid#52528Purposeserves as PCR-template to generate HR donors for C-terminal GFP-tagDepositorInserteGFP and Blasticigin resistance cassette
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only
