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pLeo-A-a2-GEMS(JAK-STAT)

Plasmid

#209110

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D3L

Plasmid

#177945

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D3L) (N )

Expression

Mammalian

Mutation

D3L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp7

Plasmid

#157688

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CoV2-N-A119S

Plasmid

#177948

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (A119S) (N )

Expression

Mammalian

Mutation

A119S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-DNMT3A PWWP

Plasmid

#59696

Purpose

Contains histone modification interacting domain (DNMT3A PWWP)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

DNMT3A PWWP (Dnmt3a Mouse)

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-GP-2-CBX7 Chromo

Plasmid

#59695

Purpose

Contains histone modification interacting domain (CBX7 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX7 Chromo (CBX7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5-V5 puro

Plasmid

#98389

Purpose

Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJH4504

Plasmid

#191702

Purpose

Pnmr-1 long::TeTx::wCherry unc-54 3' UTR C.elegans AVA/other neurons expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1 long

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

Plasmid

#113684

Purpose

EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
MSCV Puro SCRIB P305L

Plasmid

#88887

Purpose

SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

Depositor

Senthil Muthuswamy

Article

Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

Insert

SCRIB (SCRIB Human)

Use

Retroviral

Mutation

Proline to Leucine mutation @ 305th amino acid

Promoter

MSCV promoter

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pMT2 TIA1 RRM-CFP-FRBx5

Plasmid

#103783

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Tags

CFP

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CreLite

Plasmid

#131785

Purpose

AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

AAV, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only