We narrowed to 7,535 results for: ski

1. MARS-nGFP

   Plasmid

   #205234

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
   

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-2xMARS-PLD

   Plasmid

   #205237

   Purpose

   Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

   Depositor

   Jeremy Baskin

   Inserts

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   phospholipase D (PLD)
   

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET3a-FruK

   Plasmid

   #186256

   Purpose

   Overexpression of FruK for purification

   Depositor

   Liskin Swint-Kruse, Emile Van Schaftingen

   Article

   Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

   Insert

   1-phosphofructokinase (fruK E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AraC-pHG165c WT

   Plasmid

   #170114

   Purpose

   Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.

   Depositor

   Liskin Swint-Kruse

   Article

   Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

   Insert

   AraC
   

   Expression

   Bacterial

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMET17p-CCG 316

   Plasmid

   #131166

   Purpose

   For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

   Type

   Empty backbone

   Tags

   CCG

   Expression

   Yeast

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLhE_AW-pHG165 H118R

   Plasmid

   #90063

   Purpose

   Constitutive expression of a chimeric LacI:CelR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

   Insert

   LLhE_AW/H118R
   

   Expression

   Bacterial

   Mutation

   I48V/Q55A/Q60R/H118R

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLhG-pHG165a

   Plasmid

   #90039

   Purpose

   Constitutive expression of a chimeric LacI:GalR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

   Insert

   LLhG
   

   Expression

   Bacterial

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LGhG-pHG165a

   Plasmid

   #90048

   Purpose

   Constitutive expression of a chimeric LacI:GalR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

   Insert

   LGhG
   

   Expression

   Bacterial

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SMN2E7-Fluc-Nluc-reporter

   Plasmid

   #221005

   Purpose

   Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   Fluc-SMN2E7-STOP-Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Mutation

    I319V, N451D, D532N

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pV1382

    Plasmid

    #111436

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSC-ASCL1-IRES-GFP-T2A-Sox11

    Plasmid

    #182233

    Purpose

    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

    Depositor

    Chun-Li Zhang

    Article

    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

    Insert

    ASCL1-IRES-GFP-T2A-Sox11
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-TACC3-His6

    Plasmid

    #69106

    Purpose

    For bacterial expression of recombinant human TACC3 tagged with GST and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PDX1-2A-eGFP

    Plasmid

    #66964

    Purpose

    PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    pancreatic and duodenal homeobox 1 (PDX1 Human)
    

    Use

    Unspecified

    Tags

    2A-eGFP

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVG1

    Plasmid

    #111444

    Purpose

    Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 T7 Jip1

    Plasmid

    #51699

    Purpose

    JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-2xMARS-nGFP-puro

    Plasmid

    #205238

    Purpose

    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Lentiviral

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge