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pCMV6-Entry-EPHX1c.997A>C-Flag

Plasmid

#169171

Purpose

Expresses EPHX1 c.997A>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.997A>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.1212G>C-Flag

Plasmid

#169172

Purpose

Expresses EPHX1 c.1212G>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.1212G>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry-EPHX1c.1288G>C-Flag

Plasmid

#169173

Purpose

Expresses EPHX1 c.1288G>C in mammalian cells

Depositor

Bruno Feve, Jeremie Gautheron

Article

Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

Insert

epoxide hydrolase 1 (EPHX1 Human)

Tags

Flag

Expression

Mammalian

Mutation

c.1288G>C

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

Plasmid

#163175

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

Plasmid

#163174

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha6 [N229A/32R]

Plasmid

#182109

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha6 (Mouse). Derived from hybridoma N229A/32.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Alpha6 (Mus musculus) recombinant mouse monoclonal antibody (Gabra6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179433

Purpose

Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

coreHS4-TRE-cit-coreHS4-mch (DC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Kv3.3 K+ channel [N375/67R]

Plasmid

#177537

Purpose

Mammalian Expression Plasmid of anti-Kv3.3 K+ channel (Mouse). Derived from hybridoma N375/67.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv3.3 K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnc3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179426

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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