We narrowed to 18,489 results for: Met

1. pICSL1P5_crU626

   Plasmid

   #191780

   Purpose

   Cas12a guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-hSeparase-wt

   Plasmid

   #33016

   Depositor

   Marc Kirschner

   Article

   Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

   Insert

   Separase (ESPL1 Human)
   

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pRSET FLIPglu-3.2mDelta13

   Plasmid

   #13560

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16A S112A Delta13
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, S112A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. C234-E26: attB2r-Halotag-attB3

   Plasmid

   #162915

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Halotag
   

   Use

   Synthetic Biology

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. C453-E14: attB4-EF1p>-attB5

   Plasmid

   #162975

   Purpose

   Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human EF1alpha promoter
   

   Use

   Synthetic Biology

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIS.MTRIA-CCK

   Plasmid

   #184602

   Purpose

   For expression of a fluorescent sensor for CCK in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-CCK
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PRLSS-SH2-KDEL

   Plasmid

   #209716

   Purpose

   Mammalian expression of SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-SH2-KDEL
   

   Tags

   PRLSS

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPYCdKN0001

   Plasmid

   #196148

   Purpose

   Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

   Depositor

   Nicola Patron

   Article

   Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

   Insert

   9xHis_AtTGA1_HiBit
   

   Expression

   Bacterial

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB30

   Plasmid

   #122247

   Purpose

   Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDREV-0

    Plasmid

    #26750

    Depositor

    Rolf Zeller

    Article

    Osterwalder et al Nat Methods. 2010 Oct 17

    Insert

    H2B-Venus CDS (H2BC3 Human)
    

    Use

    Cre/Lox; Flp/frt; dre/rox

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET FLII275Pglu-4.6m

    Plasmid

    #13565

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A G275S-ECFP-K276R
    

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.0_LynSNAP IRES EGFP

    Plasmid

    #136625

    Purpose

    Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag (IRES EGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDGS50

    Plasmid

    #183189

    Purpose

    TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    DivIVA-GFP(tevR M0050)
    TEV-S219V
    

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbE8k-PP3359-6xHis

    Plasmid

    #83067

    Purpose

    Expresses 6xHis tagged PP3359 under pBAD regulation

    Depositor

    Matthew Chang

    Article

    Lo et al Cell Syst. 2016 Aug;3(2):133-43. doi: 10

    Insert

    PP3359
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLBC0030

    Plasmid

    #172777

    Purpose

    Level 0 module containing NT2_nYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS N-terminal eYFP (1-174)
    

    Use

    Synthetic Biology

    Mutation

    no

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLBC0031

    Plasmid

    #172778

    Purpose

    Level 0 module containing CT_nYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS N-terminal eYFP (1-174)
    

    Use

    Synthetic Biology

    Mutation

    no

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLBC0040

    Plasmid

    #172779

    Purpose

    Level 0 module containing NT2_cYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238)
    

    Use

    Synthetic Biology

    Mutation

    no

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLBC0041

    Plasmid

    #172780

    Purpose

    Level 0 module containing CT_cYFP

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS C-terminal eYFP (175-238)
    

    Use

    Synthetic Biology

    Mutation

    no

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIE046 ZF43x12-C mKate2 (TUPV1)

    Plasmid

    #138726

    Purpose

    mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF43x12-Compact

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH234-Tier1-PmPGK1-tTA

    Plasmid

    #169574

    Purpose

    Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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