We narrowed to 9,071 results for: sgRNA

Showing: 7421 - 7440 of 9071 results

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pQCi

Plasmid

#154345

Purpose

Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)

Depositor

Scott McComb

Publication

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Publication

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Expression

Mammalian

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Publication

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Publication

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCas5

Plasmid

#82378

Purpose

sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Promoter

PJ23119

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

Plasmid

#118155

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_AMBRA1#2

Plasmid

#174153

Purpose

Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_GFP

Plasmid

#141210

Purpose

empty GFP vector for cloning sgRNA or pgRNAs

Depositor

Rory Johnson

Publication

Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pB_hU6_Cas9-t2a-Puro

Plasmid

#253414

Purpose

piggyBac integration and stable expression of sgRNA and SpCas9. Based on plasmid px459 (Addgene #62988)

Depositor

Joanna Wysocka

Publication

Koska et al Nat Genet. 2026 Jul 20. doi: 10.1038/s41

Insert

Cas9

Use

CRISPR

Tags

2a-Puro and 3xFLAG

Expression

Mammalian

Promoter

Cbh

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-KLF17-CRa1-MS2

Plasmid

#251117

Purpose

sgRNA1 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Publication

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA1 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-KLF17-CRa2-MS2

Plasmid

#251118

Purpose

sgRNA2 for KLF17 activation cloned into lenti sgRNA(MS2)_puro backbone

Depositor

Ting Zhou

Publication

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA2 targeting KLF17 promoter (KLF17 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pAKanCRISPR

Plasmid

#242231

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas

Depositor

James T. Tambong

Publication

Chi et al Sci Rep. 2024 Sep 3;14(1):20517. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pX459_LIG4_Exon3

Plasmid

#225363

Purpose

pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

Depositor

Sujatha Jagannathan

Publication

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

LIG4 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSK-Spsg

Plasmid

#214351

Purpose

Expresses cloning backbone for SpCas9 sgRNA

Depositor

Yongming Wang

Publication

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9 tracrRNA

Expression

Mammalian

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bamh1

Plasmid

#203298

Purpose

For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Not1

Plasmid

#203301

Purpose

For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Publication

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Publication

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Promoter

U6

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Publication

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Publication

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTJV1ScGm-rpoB

Plasmid

#166979

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance

Depositor

Christopher Reisch

Publication

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

gentamycin-3-acetyltransferase (aac(3)-Ia )

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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