We narrowed to 18,529 results for: IRE

1. pEVOL-MmAcKRS1-PylTUUA

   Plasmid

   #182537

   Purpose

   N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion with ochre suppression

   Depositor

   Wenshe Liu

   Article

   Wu et al Chembiochem. 2012 Jul 9;13(10):1405-8. d

   Insert

   PylTUUA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProK

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14b_tev_PSGU

   Plasmid

   #59738

   Purpose

   Expresses a.a. 417-887 of human ZO-1

   Depositor

   Alan Fanning

   Article

   Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

   Insert

   zonula occludens-1 (TJP1 Human)
   

   Use

   Tags

   HIS tag and thrombin clvg

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pBabe-puro-Smad7-HA

   Plasmid

   #37044

   Depositor

   Sam Thiagalingam

   Article

   Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

   Insert

   Smad7 (SMAD7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   5'LTR

   Available since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Flag mTOR S2035T R2109A

   Plasmid

   #26606

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S2035T R2109A

   Promoter

   Available since

   Nov. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

6. WT TXNIP-FLAG

   Plasmid

   #200091

   Purpose

   WT human TXNIP with C-terminal 3xFLAG tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   TXNIP (TXNIP Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB036a

   Plasmid

   #99215

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK and PproD

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-MCM6-N-22

   Plasmid

   #54172

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

   Insert

   MCM6
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pcDNA3.1-GFP-2AFP-KDEL

   Plasmid

   #154349

   Purpose

   Mammalian expression of 2AFP

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   2AFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Geph CR3m shRNA

    Plasmid

    #121069

    Purpose

    Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Syn:eGFP-CaM-uTEV1Δ(220-242)

    Plasmid

    #135463

    Purpose

    Neuronal expression of uFLARE protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-CaM-uTEV1Δ
    

    Use

    AAV

    Tags

    eGFP, V5

    Expression

    Mammalian

    Mutation

    S219V mutation improves stability and S153N impro…

    Promoter

    Synapsin

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiGuide_GFP_sgEPAS1#1

    Plasmid

    #183119

    Purpose

    pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiGuide_GFP_sgEPAS1#2

    Plasmid

    #183120

    Purpose

    pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EPAS1-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cdk6

    Plasmid

    #1866

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk6 (CDK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D224Y

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSicoR human Dicer3

    Plasmid

    #14765

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    Dicer shRNA (DICER1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-4T-3-SH3BP1-CTD

    Plasmid

    #136649

    Purpose

    Expression of SH3BP1-CTD-GST fusion protein in E. coli

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-CTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only C-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-3-SH3BP1-NTD

    Plasmid

    #136648

    Purpose

    Expression of SH3BP1-NTD-GST fusion protein in E. coli

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1-NTD (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains only N-terminal domain

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-scrCdh2.1 GFP

    Plasmid

    #209100

    Purpose

    GFP expressing scramble of shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWUR 1+2 tetO mut89

    Plasmid

    #80102

    Purpose

    Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1 (cas1 )
    Cas2 (cas2 )
    Cas1 (cas1 )
    Cas2 (cas2 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q24H, P202Q, G241D, E276D, L297Q

    Promoter

    Available since

    Dec. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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